Traditional analysis of freezing behavior utlizes photobeams but is unreliable as the resolution provided by the photobeams is very low. FreezeScan on the other hand is a video‐based tool that provides precise motion control for accurate freezing detection.
FreezeScan accepts video taken from any angle, in a confined chamber. It precisely detects the onset and completion of a freezing behavior of a rodent. It outputs as a sequential list, the occurrences of the freezing behaviors. Further statistics can be analyzed from this output data.
FreezeScan has achieved great accuracy and has been successful in many academic and industry research labs for many types of research projects, including Fear Extinction.
FreezeScan not only detects freezing behavior accurately, but also provides a measurement of the animal’s instantaneous activity level during the experiment, in both quantitative and graphical form, making it possible to recognize startle response.
We also provide the Control Box for controlling Shock, Tone and Lights. The Control Box has the ability to control up to 64 devices. For example, if one wishes to control shock, tone and lights (3 channels) for each box, our Control Box can control 21 chambers simultaneously. If 4 channels are needed per box, then 16 boxes can be controlled simultaneously. Typically, 4 chambers are controlled using a single box.
FreezeScan has the capability to generate the program sequence for controlling various hardware. Or, if using 3rd party control units, FreezeScan has the ability to input the program sequence info or even, auto‐ detect the program intervals based on visual cues in the chamber, such as an LED light turning on/off. Thus FreezeScan can synchronously work with any control program and provide accurate freezing state results based on these intervals, also known as Interval Statistics.
FreezeScan can achieve high throughput screening as well. System architecture for high throughput screening has been designed that involves analysis of up to 4 arenas on a single system, including control of all 4 chambers’ hardware. The High‐Throughput product option is necessary to analyze more than a single arena simultaneously. The Realtime Option is necessary to perform realtime analysis where the live video feed into the computer is compressed, encoded, saved to the hard drive while simultaneously full analysis of the video is performed.
Many advanced features are incorporated, including supporting full color analysis, automatic adaptation to non‐uniform or changing environment, IR lighting, automated handling of light/dark areas, variable speed playback of specific video segment for specific detected behavior, etc.
Complete Turn‐key systems including all necessary Hardware and Software are available. Custom design of your environment to facilitate analysis, including lighting condition setup, IR/red light setup, cage enclosures, video integration, and video‐feed to computer is also available.